Q.2-Urease acts upon urea to form CO2 and NH3. How will you comment upon the specificity of Urease enzyme for its substrate? Give a brief account of enzyme specificity giving various examples.
Q.3- What is active site? How does it participate in enzyme catalysis?
Q.4- What are cofactors? How can you differentiate between cofactor, coenzyme and prosthetic group?
Q.5-Enzymes are known to increase the rate of catalysis by 100- 1000 folds or more. What are the processes involved in enzyme catalysed reactions?
Q.6.-What is activational barrier? State your answer in terms of enzyme catalyzed reactions.
Q.7-The nature works in a conservative manner. How are enzyme activities regulated as per the needs of the cells?Elaborate your answer giving suitable examples.
Q.8-What are the various factors which affect the rate of enzyme catalyzed reactions?
Or Express the significance of-
a) Rising temperature on enzyme activity
b) Variation of pH on enzyme activity
c) Rising substrate concentration for a given amount of enzyme
d) Rising enzyme concentration for a given amount of substrate
Q.9- What is the effect of compartmentalization on enzyme activity?
Or In urea cycle and in haem synthetic pathway, some of the reactions are cytoplasmic while some are mitochondrial,what is the significance of this biological compartmentalization?
Q.10- Classify enzyme inhibitors based on their mechanisms of actions.
Enlist the commercially used enzyme inhibitors that are used as poisons
Enlist the enzyme inhibitors used as pharmaceutical agents.
Q.11.-What is suicidal inhibition? Support your answer giving suitable examples
Q.12.- Differentiate between competitive and non competitive inhibition.
Q.13- Differentiate between non competitive and un-competitive inhibition.
Q.14- What is feed back inhibition? How doe sit differ from feed back regulation? Give suitable examples in support of your answer.
Q.15-What do you understand by allosteric modification? What is the ultimate mechanism involved in this mode of regulation. Give suitable examples and support your answer with diagrams.
Q.16- Methotrexate an anticancer drug is known to increase km of the enzyme dihydrofolate reductase (DHFR), for its substrate,dihydrofolate. The Vmax remains constant. What could be the possible mechanism of action of this drug? Give examples of some inhibitors which act similarly.
Q.17- What is the relationship of km with the rate of reaction and substrate concentration? Write an equation representing their mutual relationship.
Q.18-What are isoenzymes? Explain your answer giving suitable examples of clinically important isoenzymes.
Q.19-Discuss the diagnostic significance of LDH (Lactate dehydrogenase), ALP(Alkaline phosphatase) and Transaminases ?
Q.20- Enlist the significance of enzyme estimations in –
a) Liver disorders
c) Acute MI
d) Bony disorders
Q.21- Enzymes can be used as diagnostic reagents. Mention the names of the tests where enzymes participate as diagnostic reagents.
Q.22- A patient with carcinoma bladder has reported with wide-spread secondaries in the body. Which enzyme estimation would be of help in framing the diagnosis?
Q.23- Discuss the therapeutic significance of enzymes. Elaborate your answer giving examples.
Q.24-Explain how investigation of the isozymes of lactate dehydrogenase and creatine kinase in the serum of a person who had collapsed during a marathon would help to determine whether the problem was a myocardial infarction or severe skeletal muscle damage?
Q.25- Name the enzymes and coenzymes required for(a) the oxidation of ethanol to acetaldehyde and (b) the removal and replacement of the amino group from the alpha-carbon of amino acids?
Q.26-Name two coenzymes which are required for carboxylation reactions, Point out how they differ and write a reaction to illustrate the action of each coenzyme.
Q.27- What is meant by rate limiting enzyme?Give two examples of such like enzymes in support of your answer giving the reactions catalyzed by them.
Q.28- What is the unit of measuring enzyme activity? What is turn over number?
Q.29- What are zymogens? Give suitable examples.
Q.30- Enlist functional enzymes of plasma. What is the significance of measuring non functional plasma enzymes ?Please help "Biochemistry for Medics" by CLICKING ON THE ADVERTISEMENTS above!