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A clone is a population of identical organisms derived from a single parental organism. In Molecular biology- A clone is a collection of molecules or cells, all identical to an original molecule or cell. If the original cells harbored a recombinant DNA molecule in the form of a plasmid, the plasmids within the millions of cells in a bacterial colony represent a clone of original DNA molecules; these molecules can be isolated and studied. The mechanism of cloning involves , i) Cutting DNA at precise locations, ii) Joining two DNA fragments covalently, iii) Selection of a small molecule of DNA capable of self replication ,iv) Moving recombinant molecules from the test tube in to a host cell and v) Selecting or identifying those cells that contain Recombinant DNA .

Which of the following is required for joining two DNA fragments covalently?

A. DNA polymerase

B. Restriction endonucleases

C. Primase

D. Reverse transcriptase

E. DNA ligase

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