A clone is a population of identical organisms derived from a single parental organism. In Molecular biology- A clone is a collection of molecules or cells, all identical to an original molecule or cell. If the original cells harbored a recombinant DNA molecule in the form of a plasmid, the plasmids within the millions of cells in a bacterial colony represent a clone of original DNA molecules; these molecules can be isolated and studied. The mechanism of cloning involves , i) Cutting DNA at precise locations, ii) Joining two DNA fragments covalently, iii) Selection of a small molecule of DNA capable of self replication ,iv) Moving recombinant molecules from the test tube in to a host cell and v) Selecting or identifying those cells that contain Recombinant DNA .
Which of the following is required for joining two DNA fragments covalently?
A. DNA polymerase
B. Restriction endonucleases
D. Reverse transcriptase
E. DNA ligase
Check the answer at
Please help "Biochemistry for Medics" by CLICKING ON THE ADVERTISEMENTS above!