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During an extended period of exercise, the enzymes involved in the glycolytic pathway in muscle tissue actively break down glucose to provide the muscle energy. The liver, to maintain blood glucose levels, synthesizes glucose via the gluconeogenic pathway. Which of the following enzymes involved in these pathways would be most likely to exhibit Michaelis–Menten kinetics, that is, have a hyperbolic curve when plotting substrate concentration versus velocity of the reaction?

A. Fructose-1,6-bisphosphatase

B. Hexokinase

C. Lactate dehydrogenase

D. Phosphofructokinase 1

E. Pyruvate kinase


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