1. Give a brief account of each of the followings
a) P:O ratio
b) Redox Potential
c) ATP Synthase complex
d) Mitochondrial shuttles
e) Site specific inhibitors of electron transport chain
f) Uncouplers of oxidative phosphorylation
2. Give a diagrammatic representation of Electron Transport chain showing the flow of electrons, sites of ATP formation and the sites of inhibitors of Electron transport chain.
3. Show by means of a diagram the process of ATP formation by oxidative phosphorylation.
4. Show by means of diagrams the mechanisms of transfer of NADH from cytoplasm to mitochondria .
5. Differentiate between substrate level phosphorylation and oxidative phosphorylation.
6. Explain why rotenone which inhibits mitochondrial oxidation and Uncouplers (such as 2,4 DNP) are lethal to cells and organisms
7. For each of the followings, write at least three reactions where they are required as co enzymes. (a) NAD+ (b) FAD
8. Define the P:O ratio in mitochondrial oxidation. Briefly explain the different values obtained with succinate as compared to malate as the substrate.
9. Explain briefly why the oxidation of succinate to fumarate yields only 2 mol ATP per mol of succinate oxidized but oxidation of malate to oxaloacetate yields 3 mol ATP per mol of malate oxidized ?
10. Describe briefly the structure of the mitochondrial Fl-F0 ATPase (ATP synthase complex) and explain how it functions in the synthesis of ATP?
11. Explain the reason that the toxic dosage of Aspirin can cause hyperthermia?
12. Explain why the complete oxidation of I mol of Glucose in muscles yields 36 mol ATP while in liver this amounts to 38 mol ATP?
13. What is the biological advantage of uncoupling of oxidative phosphorylation?
14. What is the biochemical basis of uncoupling of oxidative phosphorylation in brown adipose tissue?
15. Catalase and Peroxidase, both act as scavengers for decomposition of H2O2, how do they differ in their actions?
16. Give a brief account of inhibitors of ATP synthesis.Please help "Biochemistry for Medics" by CLICKING ON THE ADVERTISEMENTS above!