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A 4-year-old child, on a well-child- check up, is found to have a large flank mass. A computed tomography (CT) scan demonstrates a large mass arising from the kidney, and a subsequent biopsy reveals a diagnosis of Wilm’s tumor. A pediatric oncologist starts chemotherapy containing the transcription inhibitor Actinomycin D. Which of the following statements is correct regarding transcription?

A. Eukaryotes often produce polycistronic mRNA

B. RNA polymerase requires a primer

C. The RNA chain grows in the 3′-5′ direction

D. Rho factor is critical for the initiation of RNA transcription

E. The TATA box contains a consensus sequence for the binding of RNA polymerase

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